Adult patient:
Heparin gel tube
Minimum blood volume: 5 mL |
Spot fluid: Plain bottle Minimum volume: 10 mL
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Paediatric patient:
Paediatric heparin tube Number of vials: 1 Minimum blood volume: 1.3 mL |
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Authorisation code required |
No |
No |
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24 Hr available service |
Yes
(Call extension: 3353 for express result) |
No |
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Method |
Spectrophotometry |
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Reference interval |
(Source: 1. From local population study in 2008 2. Francoise Schiele, Joseph Hanny, Joelle Hitz et al. Total Bone and Liver Alkaline Phosphatases in plasma: Biological Variations and Reference Limits. Clin Chem 1983; 29: 634 - 41) Female during pregnancy:
(Source: Klajnbard A et al. Laboratory Reference Intervals during Pregnancy, Delivery and the Early Postpartum Period. Clin Chem Lab Med. 2010 Feb; 48(2): 237 - 48.) |
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Clinical indication |
Investigation of hepatobiliary or bone disease |
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Result interpretation |
Increased levels in liver disease (particularly in association with cholestasis), bone disease (with increased osteoblastic activity, e.g. Paget’s disease), some bony metastases (especially prostate and breast), and at times in malignancy without liver or bone metastases (Regan isoenzyme)
May also be elevated in some gastrointestinal diseases or due to a macroenzyme.
Alkaline phosphatase isoenzymes (bone-specific alkaline phosphatase) are rarely necessary to identify the source of an elevated ALP.
Marked but transient elevation of ALP may be seen in children, probably attributable to viral infection.
Abnormal dentition and fragile bones with decreased ALP characterise the autosomal recessive disease hypophosphatasia.
EDTA when present chelates Ca2+ and Mg2+ and thus inactivates the ALP activity. |
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Measurement of uncertainty |
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Frequency of measurement |
Daily |