Amylase, Plasma / Urine / Fluid

 

Specimen requirement

Plasma Urine

Fluid

Adult patient:

 

 Heparin gel tube

 

Minimum blood volume: 5 mL

Spot urine:

Plain bottle

Minimum volume: 10 mL

Spot fluid:

Plain bottle

Minimum volume: 10 mL

 

Paediatric patient:

 

Paediatric heparin tube

 

Number of vials: 1

 

Minimum blood volume: 1.3 mL

Authorisation code required

No

No No

24 Hr available service

Yes

 

(Call extension: 3353 for express result)

No

No

Precaution

Please specify fluid type of the specimen.

Method

Spectrophotometry

Reference interval

Plasma:

Female: 35 - 122 U/L (at 37 °C)
Male: 33 - 120 U/L (at 37 °C)

 

(Source: from local population study in 2008)

Clinical indication

Clinical suspicion of acute pancreatitis

Result interpretation

Very high levels are found with acute pancreatitis, however, lipase is a more sensitive and specific indicator of this disorder.

 

Use of icodextrin in peritoneal dialysis may result in falsely low plasma amylase. Consider serum lipase if high index of suspicion of acute pancreatitis.

A normal amylase level does not exclude acute pancreatitis. The test is not specific, with very high levels being found in pancreatic pseudocyst, perforated duodenal ulcer and upper small bowel obstruction.

Moderately high levels are found frequently in other acute abdominal conditions.

 

High levels are also found in acute parotitis and macroamylasaemia, a clinically insignificant disorder described in 1.5% of (non-alcoholic) hospitalised adult patients.

Measurement of uncertainty

See table.

Frequency of measurement

Daily