Adult patient:
Clotted blood tube
(Serum gel separator clot activator tube)
Minimum blood volume: 5 mL
Paediatric patient:
Paediatric clotted blood tube
Number of vials: 2
Minimum blood volume: 1.6 mL
Authorisation code required
No
24 Hr available service
No
Method
Immunoassay
Reference interval
| Female | |
| 0 - 5 year-old | 18 – 37 pmol/L |
| 6 - 9 year-old | 18 – 220 pmol/L |
| > 10 year-old | |
| Follicular phase | 98 – 571 pmol/L |
| Ovulation phase | 177 – 1,153 pmol/L |
| Luteal phase | 122 – 1,094 pmol/L |
| Postmenopause | < 183 pmol/L |
| Pregnancy | |
| 1 st trimester | 563 – 11,249 pmol/L |
| 2 nd trimester | 5,729 – 69,547 pmol/L |
| 3 rd trimester | 36,810 – > 110,100 pmol/L |
| Male | |
| 0 - 9 year-old | 11 – 37 pmol/L |
| 10 - 11 year-old | 18 – 37 pmol/L |
| 12 - 14 year-old | 18 – 110 pmol/L |
| 15 - 17 year-old | 18 – 165 pmol/L |
| > 18 year-old | 37 – 184 pmol/L |
(Source:
1) Paediatric (Male and Female) and Adult Male Reference Ranges adopted from:
Roberts WL et al. Reference Information for the Clinical Laboratory. In Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 5th Edn. Burtis CA, Ashwood ER, Bruns DE eds. Elsevier Saunders 2012.
2) Adult Female Reference Ranges (5th - 95th Percentile) adopted from Manufacturer Reagent Kit Insert Estradiol III 2014 - 11, V 1.0)
Clinical indication
Diagnosis of precocious puberty
Monitoring of estrogen therapy is only possible if the drug being administered is estradiol.
Monitoring ovulation induction in in vitro fertilisation.
Assessment of women with suspected hypothalamic or pituitary disease.
Result interpretation
Increased levels indicate an endogenous or exogenous source of increased estradiol.
Due to the risk of cross reactivity, this assay should not be used when monitoring estradiol levels in patients being treated with fulvestrant.
Fulvestrant will increase the apparent concentration of estradiol in women being treated with this drug. If treatment with fulvestrant has been altered or discontinued as a result of falsely elevated estradiol results an alternate method such as LC-MS, which is not expected to show cross reactivity to fulvestrant, should be used to measure estradiol concentrations and assess the menopausal status of the patient.
Measurement of uncertainty
Frequency of measurement
Daily