Phosphate (PO4), Plasma / Urine / Fluid

Specimen requirement

Plasma

Urine Fluid

Adult patient:

 Heparin gel tube

Minimum blood volume: 5 mL

Spot urine:

Plain bottle

Minimum volume: 10 mL

24 Hr urine:

U2 bottle

Spot fluid:

Plain bottle

Minimum volume: 10 mL

 

Paediatric patient:

 

Paediatric heparin tube

 

Number of vials: 1

 

Minimum blood volume: 1.3 mL

Authorisation code required No No No
24 Hr available service

Yes

 

(Call extension: 3353 for express result)

No No
Method Spectrophotometry
Reference interval

Plasma:

Female: 0.72 - 1.43 mmol/L
Male: 0.72 - 1.39 mmol/L

 

(Source: from local population study in 2008)

 

Urine:

 

12.9 - 44.0 mmol/day

 

(Source: Robert WL et al. Reference Information for the Clinical Laboratory. In Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, 4th Edn. Burtis CA, Ashwood ER, Bruns DE eds. Elsevier Saunders 2006; 2251 - 2318)

Clinical indication

Plasma phosphate:


Assessment of patients with renal failure, metabolic bone disease, hyper- and hypo-parathyroidism

 

Urine phosphate:


Only occasionally indicated to identify phosphaturia in hypophosphataemic states.

Result interpretation

Plasma phosphate:


Increased phosphate levels are found in response to low parathyroid hormone levels (e.g. hypoparathyroidism, hypercalcaemia due to malignancy and other non-parathyroid causes) and in renal failure.

 

Decreased levels of phosphate are usually found in patients with primary hyperparathyroidism, in some cases of hypercalcaemia associated with malignancy, in renal tubular disorders and in patients using magnesium and aluminium containing antacids.

 

Levels may be decreased during prolonged intravenous therapy if phosphate supplementation is inadequate. Phosphate levels may also be decreased following a carbohydrate-rich meal, due to cellular uptake of phosphate.

 

Numerous other conditions can affect serum phosphate levels.

 

Urine phosphate:

 
Depends on diet.

 

Urinary phosphate varies with age, muscle mass, renal junction, PTH, time of day, and other factors.

 

Urinary excretion varies with diet and is essentially equivalent to dietary intake.

 

High PTH levels and renal tubular disorders will result in relatively increased phosphate excretion.

Measurement of uncertainty

See table.

Frequency of measurement Daily