Insulin, Serum

Specimen requirement Adult patient:

  Clotted blood tube (Clot activator tube) (in ice bath)

Minimum blood volume: 6 mL

Paediatric patient:

Paediatric clotted blood tube (in ice bath)

Number of vials: 2

Minimum blood volume: 1.6 mL

Authorisation code required Yes
24 Hr available service No
Precaution

If the haemoglucostix is < 2.2 mmol/L, take a simultaneous glucose and insulin level.

 

Send in ice immediately to the laboratory with concurrent plasma glucose. Phone to the laboratory (office hour 2331 / after office hour 3353) before sending the specimen.

 

Avoid haemolysis, which can lead to spuriously low value.

The presence of circulating anti-insulin antibodies and certain insulin analogues (such as aspart, detemir, glargine, glulisine and lispro) may affect the insulin result.

Method Immunoassay
Reference interval

< 29.1 mIU/L

(Source: from manufacturer's kit insert)

Footnote:
Insulin level depends on various factors including pancreatic reserve, plasma glucose, state of fasting, etc. Please interpret the insulin with concurrent plasma glucose.

Clinical indication

Investigation of fasting hypoglycaemia and insulin resistant states

Result interpretation

Increased levels and increased insulin / glucose ratios are found with pancreatic islet beta cell hyperplasia or insulinomas.

Non-insulin dependent diabetes mellitus and insulin therapy may also give high levels.

To identify self-administration of insulin as a cause of hypoglycaemia, C-peptide assay is also required.
Measurement of uncertainty See table.
Frequency of measurement 1 - 2 weeks